Work

Expression and mechanism exploration of miR-483-5p in children with IgA vasculitis with nephritis

Year: 2026

Type: article

Abstract: IgA vasculitis with nephritis (IgAVN) represents the most serious renal complication in pediatric patients with IgA vasculitis (IgAV). To characterize miR‑483‑5p expression in pediatric IgAVN patients, evaluate its potential as a diagnostic biomarker, and elucidate underlying molecular mechanisms. A total of 150 children with IgAV (55 with IgAVN, 95 with non‑IgAVN) and 60 age‑ and gender‑matched healthy controls were enrolled in this retrospective clinical study. RT-qPCR was used to detect miR-483-5p and HDAC4 mRNA expression. ROC curve analysis was conducted to assess its tentative auxiliary screening capacity for IgAV-associated renal injury. Pearson correlation analysis was used to analyze the correlation between miR-483-5p expression and clinical indicators. Logistic regression analysis was used to identify factors independently associated IgAVN. Human glomerular mesangial cells (HGMCs) were treated with lipopolysaccharide (LPS) to construct cell model. miR-483-5p was markedly downregulated in the serum and renal tissues of children with IgAVN, and serum miR-483-5p exhibited significant discriminative capacity to distinguish children with concurrent IgAVN from IgAV patients without renal involvement in this retrospective cohort. MiR-483-5p expression was negatively correlated with renal function indicators, serum IgA and inflammatory factors, and its low expression was an independently associated factor for IgAVN. In vitro, LPS downregulated miR-483-5p, promoted HGMC proliferation and inflammatory response. MiR-483-5p directly targeted and negatively regulated HDAC4. Overexpression of miR-483-5p inhibited LPS-induced HGMC proliferation and inflammatory factor secretion, while HDAC4 overexpression reversed these effects. miR‑483‑5p is downregulated in children with IgAVN and may serve as a potential non‑invasive auxiliary indicator for pediatric IgAVN. miR‑483‑5p attenuates LPS‑induced HGMC proliferation and inflammation by targeting HDAC4 in vitro; however, the in vivo mechanistic relevance and clinical utility remain preliminary and require further validation.

Source: BMC Pediatrics

DOI: 10.1186/s12887-026-07515-w

Authors: Fanghua Ming, Qing Wang, Jingru Chen

Institutions: Guangzhou Women and Children Medical Center, Guangzhou Medical University

Language: English

Topic: Renal Diseases and Glomerulopathies

Subfield: Nephrology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,090