Work

Photothermal nanomaterial-assisted multi-crRNA-enhanced one-tube RPA-CRISPR/Cas12a system for sensitive detection of Salmonella Typhimurium in milk

Year: 2026

Type: article

Abstract: Microbial Contamination serves as an important issue in dairy safety. CRISPR/Cas12a-based methods possess tremendous potential, but are limited by nucleic acid pretreatment and specialized operations. Herein, a photothermal nanomaterial-assisted enhanced One-Tube CRISPR/Cas12a system was established to detect S. typhimurium in milk . S. typhimurium was lysed through the photothermal effect to obtain genomic DNA. A multi-crRNA strategy enables rapid signal accumulation, and the whole system was integrated into a single tube via trehalose-based aqueous biphasic systems. The proposed method requires only one step of sample addition to achieve sensitive detection within 40 min, with an LOD of 10 CFU/mL, showing potential in application.

Source: npj Science of Food

DOI: 10.1038/s41538-026-01090-1

Authors: Juan Wang, Xiang Li, Jing Zhang, Chao Zhao, Xuening Shi

Institution: Jilin University

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,790