Work

DREAMS illuminates spatial DNA and RNA modification landscapes

Year: 2026

Type: article

Abstract: DNA and RNA modifications regulate gene expression and RNA processing, but their spatial organization in complex tissues remains elusive. Here we developed DNA RNA Elements Areal Mass Spectrometry (DREAMS), a mass spectrometry imaging platform that spatially maps diverse nucleic acid modifications simultaneously. Applying DREAMS to TET-deficient mouse brains (Tet1Δ/Δ and triple Tet1/2/3Δ/Δ), we uncover TET1’s unexpected role in modulating N1-methyladenosine (m1A), a pivotal RNA modification. While DREAMS reveals broad modification landscapes altered across TET knockouts, we identify TET1-mediated changes in m1A that correlate with transcriptome alterations. Our work establishes DREAMS as a transformative tool for spatial epigenomics/epitranscriptomics and suggests that TET enzymes could influence multiple DNA and RNA modifications with potential gatekeeping roles in nucleic acid regulation. Peng, Xue et al. develop a nucleic acid metabolites detection method, DNA RNA Elements Areal Mass Spectrometry (DREAMS) which enables the identification of DNA and RNA modifications, as well as spatial mapping of these modifications within tissues.

Source: Nature Cell Biology

DOI: 10.1038/s41556-026-02038-z