Work

An ode to the 16S rRNA gene: its history, importance, caveats and future in microbiome research

Year: 2026

Type: article

Abstract: The 16S rRNA gene has - and continues to - play an important role in microbiology. It's universality across prokaryotes and variation across species has allowed the sequencing of its hypervariable regions to be used to distinguish taxa within complex mixed bacterial communities. Although 16S rRNA gene sequencing has transformed our understanding of microbial communities, its use comes with important caveats and considerations, especially in light of the availability of whole-genome metagenomic sequencing. Within, we discuss the 16S rRNA gene and its important role in microbiome research, in the past, present, and future.

Source: Microbial Genomics

DOI: 10.1099/mgen.0.001823

Authors: F.J. Whelan, L.J. Hall

Institutions: University of Manchester, Norwich Research Park, Quadram Institute, University of Birmingham

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,839

Funders: Wellcome Trust, Academy of Medical Sciences, Lister Institute of Preventive Medicine, Biotechnology and Biological Sciences Research Council