Work

Protocol for short-term in vitro propagation of patient-derived group 4 medulloblastoma MBT375 tumor cells

Year: 2026

Type: article

Abstract: Durable in vitro cell culturing is an urgent unmet need for the pre-clinical development of novel group 4 medulloblastoma (G4-MB) specific therapies. Here, we present a protocol for the establishment and propagation of G4-MB tumor cells for short-term in vitro culturing. We describe brain tumor cell isolation, murine intracranial engraftment, in vitro culturing. and assay seeding. Our approach enables short-term in vitro passaging and drug screening. For long-term propagation of these G4-MB models, serial engraftment is required. For complete details on the use and execution of this protocol, please refer to Custers et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104809

Authors: Stefan Custers, Chitra Venugopal, Sheila K. Singh

Institutions: McMaster University, King's College London

Language: English

Topic: Glioma Diagnosis and Treatment

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550