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Supplementary videos - PURELIGHT: a quantitative photon-counting framework unifying intensity and lifetime imaging at video rate across detector technologies

Year: 2026
Type: supplementary-materials
Abstract: Suppmentary videos for the manuscript "PURELIGHT: a quantitative photon-counting framework unifyingintensity and lifetime imaging at video rate across detector technologies" 1. Astrocytic Ca2+ method comparison: FLIM video of the data shown in Figure4b-d (baseline and seizure) comparing the different correction methods.2. Baseline astrocyte Ca2+: FLIM video of the data shown in Figure 4g with anarrower color-scale to allow for visualization of smaller events.3. Video rate neuronal Ca2+ FLIM: Noisy and denoised 30 Hz recording of neuronswith Tq-Ca-FLITS.4. Dual area calcium imaging analog vs TCSPC comparison: Two GCaMP6fintensity channels of different cortical areas recorded simultaneously. (a) recordedwith analog mode (showing bright areas of one image become dark areas of the otherimage) and (b) photon counting (showing almost no crosstalk between areas.
DOI: 10.5281/zenodo.22123084
Authors: Velasquez Moros, Felipe, Amiet, Dorian, Skwarzynska, Daria, Meister, Rachel M, Dalvi, Urvashi, Ma, Saidong, Rupprecht, Peter, Han, Shuting, Bethge, Philipp, Krainer, Lukas, Acconcia, Giulia, Rech, Ivan, Helmchen, Fritjof, Zbinden, Paul, Saab, Aiman S, Weber, Bruno, Ravotto, Luca
Institutions: University of Zurich, ETH Zurich, University of Applied Sciences of Eastern Switzerland, Politecnico di Milano, University of Applied Sciences Rapperswil, OST - Ostschweizer Fachhochschule
Language: English
Subfield: Biophysics
Open Access status: green
OpenAlex ID: W7204538299
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