Influence of the lipid thioether modification and formulation method on lipoplex structure, stability and transfection efficiency in HEK293T cells
Year: 2026
Type: article
Abstract: ≥ 2. Cryo-TEM and SAXS revealed a multilamellar organization, with BSV107-S microfluidic lipoplexes showing larger interlamellar spacings than BSV107 or bulk-prepared systems. In HEK293T cells, transfection efficiency depended on the interplay between the lipid composition and formulation, with BSV107-S consistently outperforming BSV107 regardless of the formulation method. This improved performance correlated with increased membrane fluidity, measured by FRAP, supporting the role of thioether groups in modulating membrane dynamics. Together, these results establish structure-formulation-property relationships linking lipid chemical structure, formulation, physicochemical properties and biological performance, providing insights for the rational design of more efficient non-viral gene delivery systems.
Source: Journal of Materials Chemistry B
DOI: 10.1039/d6tb00356g
Institutions: Centre National de la Recherche Scientifique, Institut des Sciences Chimiques de Rennes, Université de Rennes, Structure Fédérative de Recherche en Biologie et Santé de Rennes, Laboratoire Chimie Electrochimie Moléculaires et Chimie Analytique, Centre de recherche Paul Pascal, École Nationale Supérieure de Chimie de Rennes, Inserm, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Nutrition, métabolismes et cancer, Laboratoire de Chimie des Polymères Organiques, Institut Polytechnique de Bordeaux
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W7204747599