LAIR1 regulated the development of chronic lymphocytic leukemia by modulating cell cycle and apoptosis
Year: 2026
Type: article
Abstract: Leukocyte Associated lmmunoglobulin Like Receptor 1 (LAIR1) has been found to be associated with chronic lymphocytic leukemia (CLL). Thus, we aimed to investigate the function of LAIR1 in cell cycle and apoptosis of CLL. Single-cell analysis was initially conducted to explore cellular signatures in CLL, and identified key cells to investigate LAIR1 expression in different cell types. The expression of LAIR1 was further analyzed between CLL and control samples in public datasets and human CLL cell line. According to the optimal cutoff value of LAIR1 expression, CLL samples were categorized into two expression groups, and survival outcomes was compared by Kaplan-Meier analysis. Subsequently, LAIR1 overexpression CLL cell lines were constructed, and validated by real time-quantitative polymerase chain reaction (RT-qPCR) and Western blot. Moreover, flow cytometry assays were performed to investigate the impact of LAIR1 on the CLL cell cycle and apoptosis. Macrophages were identified as key cells in the CLL microenvironment, exhibiting the highest expression of LAIR1. LAIR1 expression was significantly decreased in CLL group for both bioinformatics and experimental analyses, and low LAIR1 expression group had the worse prognosis. RT-qPCR and Western blot analyses confirmed that LAIR1 expression was significantly increased in CLL cell line with LAIR1 overexpression. Finally, flow cytometry analysis indicated that CLL cell with LAIR1 overexpression had shortened S-phase length and increased apoptotic rates. This study found overexpression of LAIR1 reduced S-phase length and increased apoptotic rates of CLL cells, offering novel reference for the effect of LAIR1 in CLL.
Source: Annals of Hematology
Authors: Min Min, Jun Zhang, Jingjing Wang, Yin Chen, Hewei Luan
Institution: Jiangsu University
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,590
OpenAlex ID: W7204764867
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