Figure 2 from Pulsed Electric Field Ablation Reprograms Tumor Immunity and Stimulates Germinal Center Formation in Tertiary Lymphoid Structures in Patients with Non–Small Cell Lung Cancer
Year: 2026
Type: other
Abstract: TLS are present regardless of tumor histology and are associated with increased CD8+ lymphocytes in the TME. A, Quantification of TLS in ablated tumors by histologic subtype [ADC (n = 22), mADC (n = 3), LSCC (n = 9)] shows no difference between histologies (P = 0.4653 among the three groups). P values were calculated using the Kruskal–Wallis test with Dunn's multiple comparison test. B, Violin plots demonstrating the total TLS count per zone in ablated tumors (as depicted in Fig. 1B). TLS were identified within all four TLS-relevant zones (P < 0.0001 across the four groups, with significant between-zone differences as specified in the plot), and their accumulation was more robust in the RT and Peri zone. P values were calculated using the Kruskal–Wallis test with Dunn's multiple comparison test. C, A correlation plot of total TLS with respect to the area in mm2 of the ablation zone (CDZ + TZ) indicates a weak positive correlation between total TLS and the ablation zone area (R = 0.4044, P < 0.05). P values were calculated using a nonparametric Spearman correlation. D, The percentage of CD8+ T cells trended toward being increased in ablated tumors compared with control tumors, as measured in relation to all cells within chromogenically stained tissue sections from each group. P values were calculated using the Mann–Whitney test. E, A moderate positive correlation was identified between the total TLS count and the total count of CD8+ T cells within the TLS-relevant zones in postablation resected tumors (R = 0.7027; P < 0.0001). P values were calculated using a nonparametric Spearman correlation. [Created in BioRender. Pastori, C. (2026) https://BioRender.com/0opxod7.].
Authors: Marcelo Jimenez, Beryl A. Hatton, Alicia Moreno-Gonzalez, Jeffrey S. Iding, Javier Flandes, Erik H.F.M. van der Heijden, Calvin S.H. Ng, Carlos Prieto, Cristina Teodosio, Juan A. Flores-Montero, Marta Rodríguez-González, Oderay Mabel Cedeño Diaz, Shoko Vos, Rainbow W.H. Lau, Medard E. Kaiza, William H. Moore, Alberto Orfao, Tullia C. Bruno
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7204909117