Supplementary Figures S1-S15 from Pulsed Electric Field Ablation Reprograms Tumor Immunity and Stimulates Germinal Center Formation in Tertiary Lymphoid Structures in Patients with Non–Small Cell Lung Cancer
Year: 2026
Type: supplementary-materials
Abstract: Supplementary Figure 1. CONSORT diagram of patients in the INCITE ES study (NCT04732520) and study schematic. Supplementary Figure 2. Brightfield image analysis pipeline. Supplementary Figure 3. Samples included in the brightfield IHC and multispectral fluorescent IHC analyses Supplementary Figure 4. Comparison of TLS and TLS_GC multispectral immunohistochemistry biomarkers, TLS quantification results from INCITE ES sample groups, and the association of TLS and GC formation with the pathological response to ablation. Supplementary Figure 5. Gating strategy for identification of CD4+ T cell populations by flow cytometry Supplementary Figure 6. Gating strategy for identification of CD8+ T cell populations by flow cytometry Supplementary Figure 7. Gating strategy for identification of B cell populations by flow cytometry. Supplementary Figure 8. Analysis of peripheral blood samples by flow cytometry. Supplementary Figure 9. Histopathological example of the ablation-induced cellular depletion zone (CDZ). Supplementary Figure 10. Ablation induces increased expression of stress response genes. Supplementary Figure 11. Ablation induces increased expression of genes involved in antigen recognition and T lymphocyte activation as well as increased activated and memory T lymphocytes in circulation. Supplementary Figure 12. Genes critical for GC formation and activity are significantly elevated post-ablation Supplementary Figure 13. Ablation does not result in encapsulation around the CDZ. Supplementary Figure 14. Ablation induces a humoral immune response that results in increased serum IgG1, switched memory B cells, and plasmablasts in circulation. Supplementary Figure 15. Off-target responses observed in non-ablated lesions distant from those ablated with the Aliya System in patients not part of the INCITE ES study.
Authors: Marcelo Jimenez, Beryl A. Hatton, Alicia Moreno-Gonzalez, Jeffrey S. Iding, Javier Flandes, Erik H.F.M. van der Heijden, Calvin S.H. Ng, Carlos Prieto, Cristina Teodosio, Juan A. Flores-Montero, Marta Rodríguez-González, Oderay Mabel Cedeño Diaz, Shoko Vos, Rainbow W.H. Lau, Medard E. Kaiza, William H. Moore, Alberto Orfao, Tullia C. Bruno
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7204952041