Attack on the clones? Human FOXP3 detection by PCH101, 236A/E7, 206D, and 259D reveals 259D as the outlier with lower sensitivity
Year: 2007
Type: letter
Abstract: Attack on the clones? Human FOXP3 detection by PCH101, 236A/E7, 206D, and 259D reveals 259D as the outlier with lower sensitivity We read with great interest the report by Tran and colleagues in which the authors studied the induction of FOXP3 in naive human T cells.1 The authors state that, in the absence of exogenous TGF-, only a minor subset of activated human T cells express FOXP3. In contrast, several prior reports including ours2-4 have shown FOXP3 expression on a substantial proportion of activated T cells (re-viewed in Pillai and Karandikar5). The authors attribute these findings to the “nonspecific ” staining of human T cells by clone PCH101 (eBioscience, San Diego, CA), used in multiple prior studies. The authors rely on clone 259D (Biolegend, San Diego, CA), claiming that this was similar to other clones such as 206D (Biolegend) and 236A/E7 (eBioscience), distinct from PCH101 (data mentioned but not demonstrated in the publication). If true,
Source: Blood
Authors: Vinodh Pillai, Nitin J. Karandikar
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2082382293