Work

Identification of a G-quadruplex-forming cell-free DNA fragment as a biomarker for the precise diagnosis of hepatocellular carcinoma

Year: 2026

Type: article

Abstract: cutoff of 2 was selected by maximizing the Youden index within the same cohort. The assay showed a sensitivity of 94.5% and a specificity of 90.5% for distinguishing HCC from non-HCC. Collectively, our study identifies FAM230F as a structurally stable tumor-associated cfDNA fragment and establishes a simple and scalable qPCR-based assay for HCC detection, providing a practical framework for translating cfDNA fragment analysis into clinical biomarkers.

Source: Chemical Science

DOI: 10.1039/d6sc04173f

Authors: Jianzhe Sun, Xucong Teng, Weizhi Zhang, Yicong Dai, Lianxin Liu, Jinghong Li

Institutions: University of Science and Technology of China, Tsinghua University

Language: English

Topic: Cancer Genomics and Diagnostics

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Natural Science Foundation of China, University of Science and Technology of China