Year: 2023
Type: dissertation
Abstract: BACKGROUND: Exercise-induced impairment of blood fluidity is considered to be associated with thrombosis development.However, the effects of L-arginine on blood fluidity after exercise remain unclear. OBJECTIVE:We investigated the mechanisms of impaired blood fluidity after high-intensity exercise, and examined whether L-arginine improves exercise-induced blood fluidity impairment in vitro.METHODS: Ten healthy male participants performed 15 minutes of ergometer exercise at 70% of their peak oxygen uptake levels.Blood samples were obtained before and after exercise.L-arginine and NG-monomethyl-L-arginine acetate (L-NMMA)-a nitric oxide (NO) synthase inhibitor-were added to the post-exercise blood samples.Using Kikuchi's microchannel method, we measured the blood passage time, percentage of obstructed microchannels, and the number of adherent white blood cells (WBCs) on the microchannel terrace.RESULTS: Exercise increased the hematocrit levels.The blood passage times, percentage of obstructed microchannels, and the number of adherent WBCs on the microchannel terrace increased after exercise; however, they decreased in a dosedependent manner after the addition of L-arginine.L-NMMA inhibited the L-arginine-induced decrease in blood passage time.CONCLUSIONS: High-intensity exercise impairs blood fluidity by inducing hemoconcentration along with increasing platelet aggregation and WBC adhesion.The L-arginine-NO pathway improves blood fluidity impairment after high-intensity exercise in vitro.
Author: 難波, 春地
Institution: Hiroshima University
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7208045462