Mechanisms of dietary docosahexaenoic acid‐induced protection against and amelioration of impairment of memory learning ability in Alzheimer's disease model rats
Year: 2008
Type: article
Abstract: We investigated the mechanisms of the beneficial effects of dietary docosahexaenoic acid (DHA) on the impairment of cognition ability in the amyloid beta 1- 40 (Aβ 1-40 )-infused Alzheimer's disease (AD)-model rats.After the administration of DHA to AD model rats for 12 weeks, the levels of Aβ 1-40 , and cholesterol, and the composition of fatty acids were determined in the Triton X100-insoluble membrane fractions (DIFs) of their cerebral cortex.The effects of DHA on the in vitro formation and kinetics of fibrillation of Aβ 1-40 were investigated by thioflavin T fluorescence spectroscopy, and then by transmission electron microscopy and fluorescence microscopy.Dietary DHA significantly decreased the levels of Aβ 1-40 , cholesterol and saturated fatty acids in the DIFs of AD rats.The formation of Aβ fibrils was also attenuated by their incubation with DHA, as demonstrated by the decreased intensity of thioflavin T-derived fluorescence and by electron micrographs.DHA treatment also decreased the intensity of thioflavin fluorescence in preformed-fibril Aβ peptide, demonstrating the anti-amyloidogenic and fibril-destabilizing effects of DHA.DHA not only inhibits the deposition of Aβ 1-40 in vivo but also inhibits formation and destabilization of preformed Aβ fibrils in vitro.DHA is effective in the prevention of and a radical treatment for AD.
Authors: Md. S. Hossain, 橋本, 道男, Yamashita, Shinji, 片倉, 賢紀, 田邊, 洋子, Fujiwara, Hironori, Gamoh, Shuji, Miyazawa, Teruo, Arai, Noriyuki, Shimada, Toshio, 紫藤, 治
Institutions: Tohoku University, Shimane University
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7208151444