Year: 2020
Type: article
Abstract: Cell competition is a cell–cell interaction mechanism which maintains tissue homeostasis through selective elimination of unfit cells. During early brain development, cells are eliminated through apoptosis. How cells are selected to undergo elimination remains unclear. Here we aimed to identify a role for cell competition in the elimination of suboptimal cells using an in vitro neuroepithelial model. Cell competition was observed when neural progenitor HypoE-N1 cells expressing RASV12 were surrounded by normal cells in the co-culture. The elimination through apoptosis was observed by cellular changes of RASV12 cells with rounding/fragmented morphology, by SYTOX blue-positivity, and by expression of apoptotic markers active caspase-3 and cleaved PARP. In this model, expression of juvenility-associated genes Srsf7 and Ezh2 were suppressed under cell-competitive conditions. Srsf7 depletion led to loss of cellular juvenescence characterized by suppression of Ezh2, cell growth impairment and enhancement of senescence-associated proteins. The cell bodies of eliminated cells were engulfed by the surrounding cells through phagocytosis. Our data indicates that neuroepithelial cell competition may have an important role for maintaining homeostasis in the neuroepithelium by eliminating suboptimal cells through loss of cellular juvenescence.
Source: Scientific Reports
Institution: Shiga University of Medical Science
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,870
Funders: Japan Agency for Medical Research and Development, Hoansha Foundation, Ministry of Education, Culture, Sports, Science and Technology, Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care, Kato Memorial Bioscience Foundation, Japan Brain Foundation, Japan Epilepsy Research Foundation, Epilepsiatutkimussäätiö, Japan Society for the Promotion of Science
Awards: G2231915864, Regulation of digit development by transcription factors, G2941109046, Development of preventive therapy for Alzheimer's disease by modification in expressional levels of the risk genes, G4820194847, Identification of circulating factors triggering molecular pathological processes of Alzheimer's disease, Therapeutic applications of findings with juvenility-associated genes for pediatric neurological diseases
OpenAlex ID: W3093789057