Year: 2019
Type: article
Study design: Observational Study
Abstract: Glioblastoma multiform (GBM), the most malignant brain tumor with a median survival rate of about 15 months, frequently recurs despite multimodal treatment with surgery and chemo/radiotherapies. One of the reasons why GBM is incurable is that it is impossible to remove all of GBM cells invading into the large area of brain, when patients are diagnosed. It is, therefore, crucial to identify a simple and sensitive biomarker that diagnoses GBM at its earliest stage. Exosomes are extracellular vesicles secreted by all cells including tumor cells. Since exosomes contain mRNA, microRNA (miR) and proteins specific for the cell of origin, and are relatively stable in body fluids including blood, they are attracting more attention as targets for identifying new biomarkers. Here we addressed to identify new diagnostic exosomal miR in the plasma of GBM patients, by comparing with those of healthy volunteers. Plasma samples were collected from six GBM patients and two healthy controls. We have performed RNA sequence analysis, using plasma exosomes from one preoperative GBM patient and two healthy volunteers and identified that thirty four miRs were predominantly included in the plasma exosomes of GBM patients, while forty seven ones did in those of healthy volunteers. Among the GBM plasma exosome miRs, we focused on miR-186 and miR-20a, as they have not yet shown as exosome biomarkers. Using plasma exosomes from five GBM patients and two volunteers, we performed qRT-PCR and verified the increase amount of miR-186 in the GBM plasma exosomes, whereas miR-20a showed no significant difference between GBM plasma exosomes and control ones. It should be notable that miR-186 in the GBM plasma exosomes decreased after operation, and significantly increased in recurrence. Taken together, our results identified miR-186 as a potentially useful GBMdiagnostic biomarker.
Language: Japanese
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7026326514