Soxタンパク質によるヒト内因性レトロウイルスの活性化は、カスパーゼ3経路を介して細胞アポトーシスを誘導する
Year: 2025
Type: dissertation
Abstract: Human endogenous retroviruses (HERVs) were domesticated millions of years ago as ancestral relics through germline infections and have become part of the human genome (8.3%).Over time, HERVs lost their innate ability to become virulent.We have previously reported that the transcription factor Sox2 is critical for human endogenous retrovirus-K (HERV-K) LTR5H activation and transposition in induced pluripotent stem cells.In the present study, we identified HERV-K LTR5H and LTR5B activation following Sox overexpression.In addition, we found that HERV-K Gag localized in the plasma membrane and that virus-like particles were released from Sox-expressing cells.Notably, a deformed nucleus was induced by cleaved caspase-3 in the HERV-K Gag-expressing cells.The caspase-3 inhibitors increased the number of HERV-K Gag-expressing cells by inhibiting the apoptotic pathway.Furthermore, retrotransposition of HERV-K was significantly enhanced in Sox2-expressing cells treated with caspase-3 inhibitors.Taken together, these results indicate that several Sox proteins increase HERV-K expression with cleaved caspase-3, suggesting that induction of the cell apoptotic pathway prevents genome impairment by HERV-K expression and retrotransposition.
Authors: HOSSAIN, MD JAKIR, ホセイン, エムディ ジャキール
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
Award: レトロウイルスの宿主内在化機構の解明
OpenAlex ID: W7208598560