Work

Male RORγt-transgenic mice exhibit lower body weight and an altered locomotor response to a novel environment

Year: 2026

Type: preprint

Abstract: RORγt, encoded by Rorc , is a lineage-defining regulator of T helper 17 (Th17) cell differentiation, and IL-17A signaling has been implicated in neurodevelopment and behavior. We characterized baseline behavioral phenotypes in male RORγt-transgenic mice with T-cell-biased RORγt overexpression and wild-type (WT) littermates. Mice were assessed using the resident–intruder paradigm and the light–dark box test, followed by body-weight measurement. RORγt-transgenic mice had significantly lower body weight than WT mice. No genotype differences were detected in attack latency, attack bites, attack incidence, time in the light compartment, light–dark transitions, or transition latency. In contrast, locomotor activity analyzed in 1-min bins showed a significant genotype × time interaction. Transgenic mice traveled nominally less during the first minute of the light–dark box test, although this single-bin comparison did not remain significant after correction for multiple comparisons. These findings indicate that T-cell-specific RORγt overexpression is associated with altered temporal dynamics of locomotor activity during initial exposure to a novel environment rather than with a generalized anxiety-like or aggression-related phenotype.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.08.31.748441

Authors: Tetsuya Sasaki, Aki Takahashi, Yosuke Takei

Institution: University of Tsukuba

Language: English

Topic: Psoriasis: Treatment and Pathogenesis

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green