Work

Retrieval uncertainty drives rapid consolidation of confabulated memories in retrosplenial cortex

Year: 2026

Type: preprint

Abstract: Learning to make associations in the absence of direct reinforcement, based on previous memories of reward or punishment, is an evolutionary trait quintessential for survival. Little is known about if such higher order associations (HOA) can lead to fabrication of new memories for unreal events. We probed if uncertainty during retrieval of old memory representations can trigger such confabulation. Using chemogenetics, in vivo imaging, and behavior we show that uncertainty in memory perception can not only transfer conditioning to hitherto unexperienced novel context but also can get rapidly consolidated. We find while acquisition of HOA require dorsal-CA1 parvalbumin interneuron activity in intact hippocampus, retrieval requires retrosplenial cortex (RSc). We develop an automated La rge-scale B rainwide C orrelated A ctivity M apping (LaBCAM) method to identify functional connectivity changes across different brain regions in such scenarios. LaBCAM finds that only uncertain remote retrieval engages canonical fear circuitry while regular retrieval does not. Longitudinal imaging of dendritic spines in RSc, a region involved in detection and context-dependent conflict resolution, reveals differential reorganization of spines during acquisition and rapid systems consolidation of HOA. Interestingly, we find HOA is implicated early in AD mice model (APP/PS1) and also in aged animals.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.09.01.748171