Figure 3 from Spindle Assembly Checkpoint Competency Determines Sensitivity to KIF18A Inhibition in Small Cell Lung Cancer
Year: 2026
Type: other
Abstract: KIF18A inhibition selectively impairs proliferation and mitotic spindle integrity in sensitive SCLC cells. A, Incucyte live-cell imaging analysis for cell proliferation in NCI-H841 (top) and DMS 53 (bottom) SCLC cell lines treated with different concentrations of AM-9022 for multiple timepoints. Dot plots displaying endpoint confluency at 72 hours in NCI-H841 (top) and DMS 53 (bottom) cells treated with indicated concentrations of AM-9022. P values were calculated using the Welch test. ns, not statistically significant; *, P < 0.05. B, Immunoblot analysis of KIF18A protein levels following doxycycline (DOX)–induced shRNA expression in NCI-H841 cells. Cells were cultured in the absence or presence of DOX (8 μg/mL) for the indicated times (24, 48, and 72 hours). α-Tubulin was used as the loading control. C, Representative IF images of mitotic NCI-H841 cells following DOX-induced KIF18A knockdown using shRNA_1. Cells were immunostained with α-tubulin (red) and KIF18A (green). DNA was stained with DAPI (blue). Scale bars, 10 μm. D, Quantification of metaphase cells with normal or abnormal spindle organization in NCI-H841 and DMS 53 cells cultured in the absence of doxycycline (DOX−) or in the presence of doxycycline (DOX+) to induce KIF18A knockdown. Data from both cell lines were pooled for each doxycycline condition. For each biological replicate in each cell line and condition, 100 metaphase cells were scored and classified as normal or abnormal. Percentage of cells in each category. Bars indicate the mean, and dots represent individual biological replicates from each cell line (n = 6 per condition; 3 biological replicates per cell line). *, P < 0.05, ****, P < 0.0001.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7211998983