Work

Validation of a dynamic evaporation technique for generating isoflurane/air gas mixtures standards to calibrate anesthetic gas monitors

Year: 2026

Type: article

Abstract: The dynamic evaporation and mixing technique standardized in EN 1839 (method T), originally developed to determine the flammability limits of chemical vapors, was applied to prepare isoflurane-in-air calibration gas mixtures, with air serving as the carrier gas. Isoflurane concentrations spanning 0 to 8 vol% were generated on demand by independently controlling the liquid isoflurane and air flow rates. Verification using an anesthetic gas monitor demonstrated excellent agreement between predicted and measured concentrations. A comprehensive validation study was conducted in accordance with EURACHEM guidelines, encompassing linearity, limit of detection (LOD), limit of quantification (LOQ), precision, robustness, and measurement uncertainty. The method yielded a correlation coefficient of r² = 0.9998 over the full clinical range, an LOD of 0.08 vol%, and an LOQ of 0.27 vol%. All concentrations between 0.70 and 8 vol% complied with EN 1839 uncertainty requirements, confirming the suitability of this approach for routine calibration of isoflurane gas monitors.

Source: Scientific Reports

DOI: 10.1038/s41598-026-69018-z

Authors: Aksam Abdelkhalik, Ghada Makhlouf, Randa N. Yamani

Institution: National Institute of Standards

Language: English

Topic: Scientific Measurement and Uncertainty Evaluation

Subfield: Statistics, Probability and Uncertainty

Field: Decision Sciences

Domain: Social Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,690