Figure 5 from Spindle Assembly Checkpoint Competency Determines Sensitivity to KIF18A Inhibition in Small Cell Lung Cancer
Year: 2026
Type: other
Abstract: Differential SAC signaling at kinetochores in KIF18A inhibitor–sensitive and –resistant SCLC cells. A, Representative IF images showing localization of MAD1 (green) and BUBR1 (red) at prometaphase kinetochores in monastrol-treated NCI-H841 cells. Insets, zoomed images from white boxed areas in main images. Scale bars, 5 μm for main images and 1 μm for insets. B, Representative IF images showing localization of MAD1 (green) and BUBR1 (red) at prometaphase kinetochores in monastrol-treated DMS 53 cells. Insets, zoomed images from white boxed areas in main images. Scale bars, 5 μm for main images and 1 μm for insets. C, Quantification of prometaphase kinetochore-associated MAD1 signal intensities in monastrol-treated NCI-H841 and DMS 53 cells. Bar represents mean ± SD across kinetochores (n = 108 for H841 and n = 128 for DMS 53). Kinetochores from at least 12 cells from two independent experiments were analyzed. Each dot represents a single kinetochore. D, Quantification of prometaphase kinetochore-associated BUBR1 signal intensities in monastrol-treated NCI-H841 and DMS 53 cells. Bar represents mean ± SD across kinetochores (n = 108 for H841 and n = 128 for DMS 53). Kinetochores from at least 12 cells from two independent experiments were analyzed. Each dot represents a single kinetochore. E, Quantification of the mitotic index following nocodazole treatment in NCI-H841 and DMS 53 cells. Cells were treated with DMSO, nocodazole, or nocodazole in combination with the SAC inhibitor reversine. A total of 1,500 cells were counted for each cell line and for each condition across three biological replicates. Dots indicate individual biological replicates (n = 3). Data are presented as mean ± SD. P values were calculated using two-way ANOVA with cell line and treatment as factors. P values in C and D were calculated using the Welch test. ns, not statistically significant; ****, P < 0.0001. Kts, kinetochores.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7212065975