Figure 4 from Spindle Assembly Checkpoint Competency Determines Sensitivity to KIF18A Inhibition in Small Cell Lung Cancer
Year: 2026
Type: other
Abstract: SAC-dependent mitotic arrest and cell fate outcomes following KIF18A inhibition in sensitive SCLC cells. A, Schematic timeline illustrating the experimental workflow for time-lapse imaging. MPS1i, MPS1 inhibitor. B, Representative time-lapse images of H2B-RFP–expressing NCI-H841 cells treated with DMSO, AM-9022 (0.04 μmol/L), or AM-9022 in combination with the SAC inhibitor reversine (0.05 μmol/L) showing mitotic outcome. Yellow arrows indicate mitotic cells. Scale bars, 10 μm. C, Quantification of mitotic timing in NCI-H841 cells following the indicated treatments. Mitotic timing was calculated by counting frames, each frame equivalent to 4 minutes, from nuclear envelope breakdown to anaphase onset or cell death in time-lapse images. Bars represent the median, and dots indicate individual biological replicates (n = 3). P values were calculated using the Friedman test with the Dunn multiple-comparison test. ns, not statistically significant; *, P < 0.05. D, Quantification of cell fate outcomes in NCI-H841 cells following the indicated treatments. Bar graphs show the percentage of cells undergoing normal or abnormal mitotic exit or apoptosis. Dots indicate individual biological replicates (n = 3). In C and D, 0.02 and 0.04 μmol/L denote the concentrations of AM-9022, and MPS1i denotes 0.05 μmol/L reversine, an MPS1 inhibitor.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7212083059