Metatranscriptomic (RNA-seq) taxonomy data from an iron incubation experiment during the PUPCYCLE II cruise (R/V Sally Ride) within the California Current System in May and June of 2023
Year: 2026
Type: dataset
Abstract: This dataset includes metatranscriptomic (RNA-seq) taxonomy results from an iron incubation experiment using upwelled waters sampled in the California Current System (CCS) during the PUPCYCLE II cruise with Chief Scientist Adrian Marchetti. PUPCYCLE II (Phytoplankton response to the UPwelling CYCLE) took place onboard the R/V Sally Ride from May 29th to June 10th, 2023. Freshly upwelled seawater for the incubation experiment was collected within the northern CCS and placed into designated cubitainers. Three cubitainers were immediately harvested for the initial timepoint (T0). The remaining twenty-seven cubitainers were assigned treatments: nine were unamended (Ctrl), nine were amended with 5 nM FeCl2 (+Fe), and nine were amended with 200 nM Desferrioxamine B, a strong iron chelator (DFB). Three cubitainers from each treatment were harvested for each of the three subsequent timepoints: 48 hours (T1), 168 hours (T2), and 264 hours (T3) after incubation. Approximately 2.5 to 4 liters of each sample was harvested for RNA-seq of the natural phytoplankton community, or metatranscriptomics. The dataset provides results of RNA-seq analyses describing the taxonomic compositions of each sample, with a focus on broad protist groups, mixotroph genera, and diatom genera. These results show the differences in transcriptional activity of phytoplankton taxa groups between different iron regimes, specifically the dominance of diatoms in Ctrl and +Fe treatments, and the steady presence of mixotrophs in DFB.
Language: English
Subfield: Oceanography
Field: Earth and Planetary Sciences
Domain: Physical Sciences
Open Access status: green
Funder: Division of Ocean Sciences
Award: G1220028662
OpenAlex ID: W7212150224