Figure 5 from Ribonucleotide Reductase Inhibition Triggers Ferroptosis in Genetically Defined Subsets of Non–Small Cell Lung Cancer
Year: 2026
Type: other
Abstract: RNR inhibition induces ferroptosis in oncogene-driven lung adenocarcinoma cells by increasing lipid peroxidation. A, Scatter plot representation of correlation analysis of RRM1 and ferroptosis signature gene set or RRM1 and fatty acid metabolism (FAM) signature gene sets. B–E, Bar graph representation of flow cytometry–based BODIPY/C11 staining for lipid peroxidation readout after triapine treatment (1 μmol/L, 72 hours) and gemcitabine (0.5 μmol/L, 72 hours) treatment with and without ferroptosis inhibitor, Fer-1, treatment on different lung adenocarcinoma cells. F, Bar graph representation of thiobarbituric acid reactive substance assay for measuring cellular MDA levels upon triapine treatment (1.5 μmol/L, 72 hours) in PC9, H358, and A925L cells. G, Bar graph representation of GSH assay for measuring cellular GSH levels upon triapine treatment (1.5 μmol/L, 72 hours) in PC9, H358, and A925L cells. H, Graphical representation of the effect of RNR inhibition in lung adenocarcinoma cells. For comparisons among multiple groups, one-way analysis of variance (ANOVA) was performed; between two groups, a Student t test followed by Dunnett post hoc test was performed. *, P < 0.05; **, P < 0.01; ***, P < 0.001 were considered significant [P > 0.05 was considered nonsignificant (ns)]. The error bar represents the ± standard error of the mean (SEM), and the results are a representation of n = either 2 or 3 based on the experiments.
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7212288555
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