Work

Constrained diffusion-enabled construction of a highly expressed and robust peptide asparaginyl ligase

Year: 2026

Type: article

Abstract: A combined strategy of de novo design, computational stability prediction, and activity engineering yielded FortiPAL-1, a peptide asparaginyl ligase with enhanced expression, thermostability, and catalytic efficiency.

Source: Chemical Science

DOI: 10.1039/d6sc04842k

Authors: Shuo Pang, Yuxing Hao, Jiarong Mo, Yingying He, Yinglu Cui, Bian Wu, Xinya Hemu

Institutions: China Pharmaceutical University, State Key Laboratory of Natural Medicine, Chinese Academy of Sciences, Institute of Microbiology, Beijing University of Chemical Technology

Language: English

Topic: Biochemical and Structural Characterization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, National Key Research and Development Program of China

Awards: G2966214039, G357056235, G5775531793, G872381303

OpenAlex ID: W7213230360

FWCI:

Cites: 64

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Related to: 10