Extensive disruption of HoxA/D genes does not cause obvious defects in anterior vertebral patterning in zebrafish
Year: 2026
Type: preprint
Abstract: Hox genes play central roles in specifying positional identities along the vertebrate anterior-posterior axis. In mice, genetic analyses have demonstrated that Hox genes distributed among the four Hox clusters contribute to vertebral patterning, with extensive functional redundancy among paralogous genes. Our previous genetic analysis in zebrafish identified important roles for HoxB- and HoxC-related genes in specifying anterior vertebral identities, whereas the contributions of HoxA- and HoxD-related genes remained unresolved. Here, we examined adult zebrafish carrying extensive combinations of hoxaa, hoxab , and hoxda cluster deletions and generated five-gene homozygous mutants carrying frameshift mutations in hoxa3a, hoxa4a, hoxa5a, hoxd3a , and hoxd4a . X-ray micro-CT analysis revealed no obvious anterior vertebral transformations in either the compound cluster mutants or the five-gene mutants. Together with our previous findings, these results suggest that vertebral patterning functions are distributed unevenly among zebrafish hox clusters, with a predominant contribution from HoxB/C-related genes.
Institutions: Saitama University, National Institute of Genetics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Award: ゼブラフィッシュHoxコードの発生遺伝学解析
OpenAlex ID: W7213254179