Work

High confidence HIF-1 target genes for quantifying the activation of the HIF-1-mediated hypoxia response

Year: 2026

Type: article

Abstract: The hypoxia-inducible factor 1 ( HIF-1 )-mediated hypoxia response is an evolutionarily conserved stress response that enables adaptation to conditions of low oxygen and mitochondrial dysfunction. This pathway has been shown to affect both stress resistance and longevity. HIF-1 activity is regulated by EGL-9 , VHL-1 , and RHY-1 and induces the expression of genes involved in metabolism and cellular resilience. To find genes that can be used to monitor HIF-1 activation, we compared HIF-1 modulated genes identified by multiple RNA-seq experiments and HIF-1 target genes identified by ChIP-seq experiments. We identified high-confidence HIF-1 target genes that provide robust markers of pathway activation.

Source: PubMed Central

DOI: 10.17912/micropub.biology.002304

Authors: Camille AH Desnos, Aura A Tamez Gonzalez, Jeremy M Van Raamsdonk

Language: English

Topic: Cancer, Hypoxia, and Metabolism

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, Canadian Institutes of Health Research, Natural Sciences and Engineering Research Council of Canada, Fonds de Recherche du Québec - Santé

Award: Caenorhabditis Genetics Center