Work

Optimized Phenol–Chloroform–Isoamyl DNA Extraction Protocol for Single Fish Eggs

Year: 2026

Type: article

Abstract: ) by modifying buffer volumes, incubation times, and washing steps, following prior micropuncturing of eggs on a glass slide. DNA quality is confirmed by spectrophotometry, PCR amplification of the mitochondrial COI gene, electrophoresis, and Sanger sequencing. This method provides a low-cost and effective approach for species identification from individual fish eggs. Key features • Effective and low-cost DNA extraction method from fish eggs. • Uses only standard reagents and equipment, allowing wide application in all laboratories. • High PCR efficiency.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5839

Authors: Iva Vrgo&ccaron;, Ivana Plei&cacute;, Klara Ivaniševi&cacute;, Tanja Bubi&cacute;

Institution: Institute of Oceanography and Fisheries

Language: English

Topic: Environmental DNA in Biodiversity Studies

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: diamond

Funder: Hrvatska Zaklada za Znanost

Awards: G3408410167, G4268986784, Enhancing Environmental Performance of Net-Pen Marine Aquaculture