Regenerative endodontic therapy in mature necrotic permanent teeth using human amnion - Chorion membrane as an extracellular matrix scaffold: A case series
Year: 2026
Type: article
Abstract: Dental pulp regeneration represents a paradigm shift in endodontics, aiming to restore the vitality and function of the pulp-dentin complex rather than replacing it with inert materials. This case series evaluates the use of dehydrated human amnion-chorion membrane (dHACM) as a scaffold in regenerative endodontic procedures for mature necrotic permanent teeth. Patients aged 18-40 years with necrotic maxillary anterior teeth exhibiting periapical radiolucency were treated using a standardized protocol involving canal disinfection, intracanal calcium hydroxide medication, and induction of apical bleeding. dHACM was placed over the formed blood clot as a bioactive scaffold, followed by Biodentine and definitive coronal restoration. Clinical and radiographic follow-up demonstrated significant periapical healing, reduction in radiolucency, and increased bone density. A delayed yet positive response to pulp sensibility testing was observed, suggesting partial restoration of pulp vitality. No adverse effects or undesirable calcific changes were noted. The favorable outcomes may be attributed to the rich composition of dHACM, including growth factors, cytokines, and extracellular matrix proteins that promote angiogenesis, cellular proliferation, differentiation, and immunomodulation. Within the limitations of this case series, dHACM appears to be a promising scaffold for regenerative endodontics; however, further long-term clinical studies are necessary to establish its efficacy and standardize its clinical application.
Institution: Bapuji Dental College and Hospital
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7213360187