Year: 2026
Type: article
Study design: Observational Study
Abstract: Gastric infection by Helicobacter pylori ( H. pylori ) is associated with extra-gastric pathologies. H. pylori can promote these pathologies by generating extracellular vesicles (Hp-EVs), which cross biological barriers and access other tissues through the circulation. However, the presence of Hp-EVs in the human circulation has not been demonstrated. We isolated and characterized EVs from serum of non-infected and infected patients, and used immunoblots (IB) to identify markers from host cells EVs (h-EVs) and Hp-EVs. We also assessed the predictive capacity of IB with an anti-Urease subunit B (UreB) antibody to identify true positive cases of H. pylori infection. Finally, we compared the diagnostic utility of IB-UreB with other detection methods of H. pylori in blood samples. Thirty symptomatic individuals underwent endoscopy, gastric biopsy and blood tests. Rapid Urease test (RUT), pathological analysis and Gastropanel ® (GP) classified 16 cases (53.3%) as H. pylori positive. EV markers CD63 and CD81 were detected in all the isolated EVs from the serum of these patients. Moreover, UreB+ EVs were detected in 62.5% of H. pylori -infected patients, providing the first evidence of circulating Hp-EVs in human serum. Among the blood biomarkers evaluated, IB-UreB+ proved to be the best method for diagnosing H. pylori -positive cases. Furthermore, combining IB-UreB+ with GP increased its diagnostic value. Our findings provide the first evidence of circulating Hp-EVs in human serum, thereby supporting a mechanism for systemic dissemination of bacterial virulence factors. Additionally, their detection in blood samples may enable minimally invasive diagnosis of H. pylori infection.
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
Awards: A DUAL ROLE FOR KCNQ1OT1 IN THE INDUCTION AND DISRUPTION OF SENESCENCE IN THE PREMALIGNANCY OF GASTRIC CANCER, G1420332903, MECHANISMS TRIGGERING AVB3 INTEGRIN EXPRESSION IN ASTROCYTE REACTIVITY, G1824075703, CONTROLLING NEUROTOXICITY OF REACTIVE ASTROCYTES IN INFLAMMATORY CONDITIONS BY ALTERING ENDO-LYSOSOMAL TRAFFICKING, G2288032780, G3180033168, TRANSLATIONAL REGULATION OF THE ANTIAPOPTOTIC PROTEIN SURVIVIN BY CONTROL ELEMENTS IN THE 5' UNTRANSLATED REGION (5'UTR), HELICOBACTER PYLORI EXACERBATES NEURODEGENERATION THROUGH UREASE-RICH OUTER MEMBRANE VESICLES THAT INCREASE ASTROGLIOSIS, G4382588409, CENTRO AVANZADO DE ENFERMEDADES CRONICAS/ ADVANCED CENTER FOR CHRONIC DISEASES (ACCDIS), G652983587, CAVEOLIN-1-INDUCED CHANGES IN CANCER CELL METABOLISM AND EXOSOME COMPOSITION AS MEDIATORS OF M2 MACROPHAGE POLARIZATION TO PROMOTE TUMOR ANGIOGENESIS, EXPLORING NIR-II PLASMON NANOPARTICLES FOR THE PHOTOTHERMAL DISAGGREGATION OF TOXIC AMYLOIDS AND THEIR DETECTION BY ALTERNATIVE METHODOLOGIES AND ADMINISTRATION ROUTES, ROLE OF MICRORNAS IN THE PATHOGENESIS OF GASTRIC CARCINOMA AND DEVELOPMENT OF NON-INVASIVE STRATEGIES FOR EARLY DETECTION, CENTRO DE ESTUDIOS AVANZADOS DE ENFERMEDADES CRONICAS (ACCDIS), THE ROLE OF ADIPOCYTE DERIVED EXTRACELLULAR VESICLES CONTAINING CAVEOLIN-1 IN PROMOTING TUMOR ANGIOGENESIS, IMPLEMENTACION DE UNA PLATAFORMA PARA CARACTERIZAR NANOESTRUCTURAS (TAMANO POTENCIAL ZETA CONCENTRACION Y FLUORESCENCIA) DE INTERES EN DIVERSAS DISCIPLINAS., G8794338893, G99091514
OpenAlex ID: W7213368452