Formulation and Development of Cephalandra indica Gold Nanoparticle Loaded Gastro-retentive Gel for Stress Induced Cardiomyopathy
Year: 2026
Type: article
Abstract: Stress-induced cardiomyopathy (Takotsubo syndrome) is a transient cardiac disorder associated with intense emotional or physical stress, catecholamine excess, oxidative imbalance, and inflammation.The present study aimed to develop and evaluate a gastro retentive in situ gel incorporating green-synthesized gold nanoparticles (AuNPs) using Cephalandra indica leaf extract.The methanolic extract showed concentration-dependent antioxidant activity in DPPH and hydrogen peroxide assays and exhibited significant anti-inflammatory effects in protein-denaturation and membrane-stabilization assays.Green-synthesized AuNPs showed a surface plasmon resonance peak at 525 nm, spherical morphology, a mean particle size of 39.8 ± 0.8 nm, and a zeta potential of -31.0 mV, confirming the formation of stable nanoparticles.Eight raft-forming in situ gel formulations containing sodium alginate, HPMC K100M, xanthan gum, and AuNPs were developed and evaluated for floating characteristics and drug release.Floating lag time ranged from 4.52 ± 2.78 to 56.17 ± 3.62 min, whereas cumulative release after 6 h varied from 65.50 ± 1.98% to 87.07 ± 0.85%.The optimized formulation exhibited prolonged gastric retention and controlled release characteristics.In-vivo stress-induced cardiomyopathy model, treatment with the optimized formulation significantly reduced serum markers CK-MB and troponin T levels and attenuated inflammatory and oxidative stress markers, including C-reactive protein, myeloperoxidase, lipid peroxidation, and nitric oxide.Restoration of myocardial antioxidant status was also observed compared with the untreated stress group.Overall, the findings suggest that the C. indica-AuNP gastro-retentive in situ gel is a promising sustained release approach for cardio protective management of stress-induced myocardial injury.Further validation requires optimized formulation, ethical compliance, and comprehensive animal studies.
Authors: Smita Jain, Manju Prajapati
Language: English
Subfield: Pharmaceutical Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7213416146