DBiT-seq
Year: 2026
Type: standard
Abstract: Deterministic Barcoding in Tissue for spatial omics sequencing (DBiT-seq) simultaneously maps mRNA transcripts and protein expression within intact tissue sections using microfluidic co-delivery of DNA barcodes. Spatially indexed barcodes are deposited in a deterministic grid pattern onto the tissue, capturing both RNA and antibody-DNA conjugates, which are subsequently sequenced to yield co-registered transcriptomic and proteomic profiles. This approach provides paired spatial gene expression and protein abundance data at near-cellular, pixel resolution.
Authors: Fisher, Stephen A, Hardi, Josef, Morgan, Richard, Musen, Mark A, Silverstein, Jonathan C, Nordgren, Erik, Kant, Peter M, Honick, Brendan John, O'Connor, Martin J, Rosario, Jean G, Gehlenborg, Nils, Blood, Philip D, Ahn, Kyung Jin, Anderton, Christopher R, Bandyopadhyay, Shovik, Bedi, Kenneth C, Brusko, Maigan, Campbell-Thompson, Martha, Carson, James, Carver, Chase M, Chen, Jing, Corbett, Anthony M, Cuaycal, Alexandra E, Cuda, Penny, Diep, Dinh, Domanskyi, Sergii, Donahue, Sean, Duffy, Michael P, Eadon, Michael T, Fan, Jean, Farrow, Melissa A, Fisch, Kathleen M, Flynn, William F, Fulcher, James M, Ghose, Soumya, Ginty, Fiona, Gonçalves, Joana P, He, Yongqun, Hu, Po, Jain, Sanjay, Karathanos, Thomas V, Kaushal, Madhurima, Kruse, Angela RS, Kwon, Yumi, Lake, Blue B, Lardenoije, Roy, Lin, Shin, Lin, Yiing, Lindsay, Scott A, Lu, Peiran, Mathews, Clayton, McDonough, Elizabeth, Melo Ferreira, Ricardo, Monte, Emma M, O'Neill, Kathleen, Pang, Minxing, Parast, Mana, Pei, Liming, Peters, Samuel, Pillai, Ajay, Pryhuber, Gloria, Qin, Ling, Rajbhandari, Presha, Ruffalo, Matthew M, Sarder, Pinaki, Saunders, Diane C, Schneider, Kevin, Shi, Lingyan, Sivajothi, Santhosh, Smith, David, Spraggins, Jeff M, Stanley, Valentina, Tan, Kai, Thadi, Anusha, Tian, Hua, Turner, Morgan L, Vlachos, Ioannis S, Winfree, Seth, Wu, Pei-Hsun, Zemaitis, Kevin J, Zhou, Mowei, Zhu, Chenchen
Institutions: University of Pennsylvania, Stanford University, University of Pittsburgh, Otsuka (United States), Pittsburgh Supercomputing Center, Carnegie Mellon University, Harvard University, Children's Hospital of Philadelphia, Pacific Northwest National Laboratory, Brigham and Women's Hospital, University of Florida, The University of Texas at Austin, Mayo Clinic, University of Rochester Medicine, University of Rochester, University of California San Diego, Altos Labs, The Jackson Laboratory for Genomic Medicine, Indiana University School of Medicine, Johns Hopkins University, Vanderbilt University, Delft University of Technology, University of Michigan, Washington University in St. Louis, The Ohio State University, Emory University, University of Minnesota, National Institutes of Health, Columbia University, Northwestern University, Lurie Children's Hospital, Buck Institute for Research on Aging, Columbia University Irving Medical Center, Broad Institute, Beth Israel Deaconess Medical Center, University of Nebraska Medical Center, Zhejiang University
Language: EN
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7213583040
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