Second Harmonic Generation
Year: 2026
Type: standard
Abstract: Second Harmonic Generation (SHG) microscopy is a nonlinear optical imaging technique that produces label-free, highly specific contrast from non-centrosymmetric biological structures—most notably fibrillar collagen. When pulsed laser light interacts with ordered molecular assemblies such as collagen fibrils, photons at exactly half the excitation wavelength are coherently emitted, providing structural specificity without the use of exogenous labels or stains. SHG microscopy enables high-resolution, label-free imaging of extracellular matrix architecture and collagen fiber organization in biological tissues.
Authors: Fisher, Stephen A, Hardi, Josef, Morgan, Richard, Musen, Mark A, Silverstein, Jonathan C, Nordgren, Erik, Kant, Peter M, Honick, Brendan John, O'Connor, Martin J, Rosario, Jean G, Gehlenborg, Nils, Blood, Philip D, Ahn, Kyung Jin, Anderton, Christopher R, Bandyopadhyay, Shovik, Bedi, Kenneth C, Brusko, Maigan, Campbell-Thompson, Martha, Carson, James, Carver, Chase M, Chen, Jing, Corbett, Anthony M, Cuaycal, Alexandra E, Cuda, Penny, Diep, Dinh, Domanskyi, Sergii, Donahue, Sean, Duffy, Michael P, Eadon, Michael T, Fan, Jean, Farrow, Melissa A, Fisch, Kathleen M, Flynn, William F, Fulcher, James M, Ghose, Soumya, Ginty, Fiona, Gonçalves, Joana P, He, Yongqun, Hu, Po, Jain, Sanjay, Karathanos, Thomas V, Kaushal, Madhurima, Kruse, Angela RS, Kwon, Yumi, Lake, Blue B, Lardenoije, Roy, Lin, Shin, Lin, Yiing, Lindsay, Scott A, Lu, Peiran, Mathews, Clayton, McDonough, Elizabeth, Melo Ferreira, Ricardo, Monte, Emma M, O'Neill, Kathleen, Pang, Minxing, Parast, Mana, Pei, Liming, Peters, Samuel, Pillai, Ajay, Pryhuber, Gloria, Qin, Ling, Rajbhandari, Presha, Ruffalo, Matthew M, Sarder, Pinaki, Saunders, Diane C, Schneider, Kevin, Shi, Lingyan, Sivajothi, Santhosh, Smith, David, Spraggins, Jeff M, Stanley, Valentina, Tan, Kai, Thadi, Anusha, Tian, Hua, Turner, Morgan L, Vlachos, Ioannis S, Winfree, Seth, Wu, Pei-Hsun, Zemaitis, Kevin J, Zhou, Mowei, Zhu, Chenchen
Institutions: University of Pennsylvania, Stanford University, University of Pittsburgh, Otsuka (United States), Pittsburgh Supercomputing Center, Carnegie Mellon University, Harvard University, Children's Hospital of Philadelphia, Pacific Northwest National Laboratory, Brigham and Women's Hospital, University of Florida, The University of Texas at Austin, Mayo Clinic, University of Rochester Medicine, University of Rochester, University of California San Diego, Altos Labs, The Jackson Laboratory for Genomic Medicine, Indiana University School of Medicine, Johns Hopkins University, Vanderbilt University, Delft University of Technology, University of Michigan, Washington University in St. Louis, The Ohio State University, Emory University, University of Minnesota, National Institutes of Health, Columbia University, Northwestern University, Lurie Children's Hospital, Buck Institute for Research on Aging, Columbia University Irving Medical Center, Broad Institute, Beth Israel Deaconess Medical Center, University of Nebraska Medical Center, Zhejiang University
Language: EN
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7213585190
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