Work

PIC-RNA-seq for Region-Specific Transcriptomic Analysis of Chicken Limb Bud Progenitors

Year: 2026

Type: article

Abstract: genes, in LPCs, suggesting the initiation of their regional patterning program. This workflow enables visualization of dynamic changes in the gene expression profile of LPCs and should also be applicable to other tissues in avian embryos. Key features • An optimized PIC-RNA-seq protocol for the somatic lateral plate mesoderm (sLPM) and limb bud progenitor cells (LPCs) in chicken embryos. • Applicable to chicken embryos without the need for tissue-specific transgenic reporter lines. • Robust generation of high-quality RNA-seq datasets with sufficient sequencing depth, unique molecular identifiers (UMIs), and detected genes for downstream analyses. • Enables direct comparison between pre-specified sLPM and specified LPCs, allowing the detection of transcriptional changes accompanying early LPC specification.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5843

Authors: Seung Kwon, Zhaonan Zou, Seiko Watanabe, Mizuki Honda, Shinya Oki, Yuji Atsuta

Institutions: Kyushu University, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto University, Hiroshima University

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond