Work

Analysis code for single-cell RNA sequencing of a CRISPR-engineered endogenous Drosophila tauopathy model

Year: 2026

Type: book

Abstract: Analysis code supporting the study “Transcriptional programs mediating neuronal toxicity and altered glial–neuronal signaling in a Drosophila knock-in tauopathy model.” The repository contains scripts and notebooks for Cell Ranger preprocessing, Scrublet doublet detection, Seurat-based single-cell RNA-seq analysis, trajectory analysis, FlyPhoneDB, pySCENIC, OmicsIntegrator, and generation of selected manuscript results. Associated sequencing data are available from GEO under accession GSE223345.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.22237380

Authors: Dong, Xianjun, Genomics and Bioinformatics Hub

Institutions: Yale University, Aligning Science Across Parkinson's, Brigham and Women's Hospital

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Aligning Science Across Parkinson’s

Award: G978450271