Green Extraction of Antioxidants from Lentinula edodes Using 2- Hydroxyethylammonium Acetate Ionic Liquid assisted by an ultrasonic bath
Year: 2026
Type: preprint
Abstract: Shiitake mushrooms ( Lentinula edodes ) are valued for their nutritional and therapeutic properties and are an important source of bioactive antioxidant compounds. However, the application of ionic liquids (ILs) for extracting these compounds from fungal matrices remains underexplored. This study evaluated 2-hydroxyethylammonium acetate as a green solvent for extracting antioxidant and phenolic compounds from shiitake mushrooms. Extraction was conducted by solvent–biomass contact followed by vortex agitation and ultrasonic treatment. A 2³ factorial design was employed to investigate the effects of ionic liquid concentration (0.4–2.0 mol L⁻¹), temperature (25–60°C), and ultrasonic extraction time (30–60 min). Total phenolic content was quantified using the Folin–Ciocalteu method, and antioxidant capacity was assessed via DPPH and ABTS radical scavenging assays. Spectrophotometric results indicated total phenolic contents ranging from 18.43 to 32.71 mg 100 g⁻¹. Antioxidant activity varied between 0.09 and 0.73 mM TE g⁻¹ for DPPH and from 1.48 to 2.20 mg 100 g⁻¹ for ABTS. Statistical analysis demonstrated that ionic liquid concentration and temperature significantly affected (p < 0.05) total phenolic content and DPPH activity, whereas no significant effects were observed for ABTS under the studied conditions. The findings confirm that 2-hydroxyethylammonium acetate is an effective and environmentally favorable solvent for recovering antioxidant compounds from shiitake mushrooms. This approach contributes to sustainable biomass valorization and supports the development of greener extraction strategies with potential applications in food, nutraceutical, and pharmaceutical sectors
Source: Research Square
Language: English
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7213938444