Work

Whole-brain AAV Toolbox BARseq Datasets

Year: 2025

Type: other

Abstract: Our goal is to develop enhancer virus tools that can provide access to select cell classes, subclasses, and types brain-wide. Enhancer-adeno-associated viruses (enhancer AAVs) will be evaluated for specificity and completeness of cell-type labeling using a standardized BARseq spatial transcriptomics approach. Select 20-micron sections from each AAV-injected mouse brain were subjected to in situ sequencing to detect expression of endogenous cell-type marker genes as well as exogenous virally-encoded mRNA (e.g., SYFP). For all datasets, we performed cell segmentation, registration to mouse common coordinate framework, and cell type mapping to the whole mouse brain taxonomy. This data can be used to assess the specificity and completeness of AAV vector expression within cell types and brain regions.

Source: Brain Image Library

DOI: 10.35077/g.1190

Authors: Tasic, Bosiljka, Ting, Jonathan, O'Neill, Tulip, Ben-Simon, Yoav, Hunker, Avery, Waters, Jack, Taormina, Michael J., Hastings, Sam, Allen Institute

Institution: Allen Institute for Brain Science

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institutes of Health

Award: Enhancer AAV toolbox for cell classes and subclasses in the brains of mouse, macaque and marmoset.

OpenAlex ID: W7214000604

Cites:

Cited by:

Related to: