Work

Whole-brain AAV Toolbox Light Sheet Datasets

Year: 2025

Type: other

Abstract: Our goal is to develop enhancer virus tools that can provide access to select cell classes, subclasses, and types brain- wide. Enhancer-adeno-associated viruses (enhancer AAVs) will be screened for labeling specificity by whole-brain light sheet imaging. Datasets are produced by axially-swept light sheet microscopy of whole mouse brains injected with enhancer AAVs using the SmartSPIM (LifeCanvas) platform. Cell detection and anatomical placement are performed on the full-resolution three- dimensional images. In addition, we generate a series of maximum intensity projections (MIPs), representing 30 µm of depth at 100 µm spacing from the full-resolution three-dimensional images, that can be quickly and consistently visually evaluated for overall expression strength and distribution.

Source: Brain Image Library

DOI: 10.35077/g.1189

Authors: Tasic, Bosiljka, Ting, Jonathan, Ben-Simon, Yoav, Hunker, Avery, Waters, Jack, Taormina, Michael J., Hastings, Sam, Lusk, Nicholas

Institution: Allen Institute for Brain Science

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: Enhancer AAV toolbox for cell classes and subclasses in the brains of mouse, macaque and marmoset.

OpenAlex ID: W7214024633

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