Work

Serial Cryosectioning for the Spatial Transcriptomics of Plant Tissues

Year: 2026

Type: article

Abstract: thalli. Key features • Optimized cryo-embedding and cryosectioning workflow for small plant tissues for 10x Visium spatial transcriptomics. • A streamlined computational workflow for serial-section processing, spatial-domain annotation, 3D reconstruction, and developmental trajectory analysis.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5844

Authors: Chi- Wu, Kun- Hsieh, Chun- Yu, Yi- Chen, Shu- Chou, Ting- Wu, Chin-Min Ho, Shu- Wu, Mei-Yeh Lu, Wen- Li

Institutions: Institute of Plant and Microbial Biology, Academia Sinica, Biodiversity Research Center, Academia Sinica, Academia Sinica, Research Center for Information Technology Innovation, Academia Sinica, University of Chicago

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W7214037221

FWCI:

Cites: 12

Cited by:

Related to: 10