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High-throughput optimized prime editing-mediated endogenous protein tagging for pooled imaging of protein localization - Supplementary datasets related to Figure 6g

Year: 2026

Type: other

Abstract: Data related to Figure 5g in the manuscript "High-throughput optimized prime editing-mediated endogenous protein tagging for pooled imaging of protein localization".Representative 10-cell albums for all confidently tagged genes (SupplementaryData2-3) and tagging spacers (SupplementaryData4-7). Single cell images were randomly selected for cells in UMAP bins above the median density of cell counts for a given pegRNA spacer. Nuclear areas are tinted blue while retaining the underlying intensity pattern of the NG11-tag channel and are segmented based on the DAPI channel. Cellular segmentation outlines are based on the phalloidin channel.

Source: Figshare

DOI: 10.6084/m9.figshare.33945589

Authors: Henry Sanchez, Deepak C. Mani, Tomer Lapidot, Ophir Shalem

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7214040184

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