A multiplex serological assay for quantification of arbovirus antibody kinetics and neutralizing antibodies
Year: 2026
Type: article
Abstract: Arboviruses such as dengue virus (DENV) and chikungunya virus (CHIKV) pose major global health threats. To support serological surveillance and study cross-reactivity, an in-house multiplex bead-based immunoassay was developed to measure IgG responses against a wide panel of orthoflavivirus and alphavirus antigens. Samples from Senegal, France, the Philippines, and Bangladesh enabled analyses to be carried out in endemic and non-endemic regions. IgG kinetics following DENV1 and CHIKV infections revealed virus-specific temporal profiles and identified antigens, such as CHIKV E2 and VLP, as well as DENV1 EDIII and SNAP-tagged EDIII, as promising markers for serosurveillance. A two-step Random Forest model was used to classify PRNT-positive samples and predict PRNT titers. CHIKV showed a strong correlation between IgG and PRNT titers (R² = 0.71), while DENV1 showed weaker performance (R² = 0.42). These results support the use of multiplex serology for arbovirus surveillance and highlight the limitations of using binding antibodies to predict neutralisation, particularly for orthoflaviviruses.
Source: PLoS neglected tropical diseases
Authors: Laura Garcia, Victor Yman, Marie-Fabrice Gasasira, Françoise Donnadieu, Jason Rosado, Lucie Cappuccio, Damien Hoinard, Philippe Desprès, Stéphane Petres, Jean-Claude Manuguerra, In-Kyu Yoon, Taweewan Hunsawong, Maria Theresa P. Alera, Emily S. Gurley, Nikos Vasilakis, Scott C. Weaver, Ziaur Rahman, Ilaria Dorigatti, Chris Drakeley, Inès Vigan-Womas, Makhtar Niang, Henrik Salje, Jessica Vanhomwegen, Michael White
Institutions: Institut Pasteur, Université Paris Cité, Karolinska Institutet, Stockholm South General Hospital, Centre National de la Recherche Scientifique, Inserm, Processus Infectieux en Milieu Insulaire Tropical, Institut de Recherche pour le Développement, Biologie Moléculaire Structurale et Processus Infectieux, International Vaccine Institute, Armed Forces Research Institute of Medical Science, Walter Reed Army Institute of Research, Johns Hopkins University, International Centre for Diarrhoeal Disease Research, The University of Texas Medical Branch at Galveston, Imperial College London, London School of Hygiene & Tropical Medicine, Institut Pasteur de Dakar, University of Cambridge
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,670
Awards: G1144940652, G1979782309, G282427092, G2872175397, G3256177976, Reconstructing the transmission intensity of arboviruses across Africa and the impact of interventions in a changing climate, Reconstructing the transmission intensity of arboviruses across Africa and the impact of interventions in a changing climate, Institut Convergences pour l’étude de l’Emergence des Pathologies au Travers des Individus et des populatiONs, G5420063140, G6907559088, G7240651115, G7376787254, G7404307812, G7422929747, G7540829840, Algorithms and multiplex assays for integrated serological surveillance of malaria and neglected tropical diseases, G8670608220, G9000380724
OpenAlex ID: W7214107456