Work

Antiangiogenic Drug Testing Using Proangiogenic and Hypoxia Zebrafish Model

Year: 2026

Type: article

Abstract: mutant zebrafish line with constitutive activation of the hypoxia signaling pathway using CRISPR-CAS9 mutagenesis. • The generated model exhibits extensive ectopic neovascular branching and abnormal vascular growth resembling pathological angiogenesis observed in solid tumors. • Mimics the chronic hypoxic microenvironment commonly associated with cancer progression and angiogenesis. • Provides an economical alternative to mammalian tumor models for early-stage antiangiogenic drug testing.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5846

Authors: S. Vinoth, Kirankumar Santhakumar

Institution: SRM Institute of Science and Technology

Language: English

Topic: Zebrafish Biomedical Research Applications

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: SRM Institute of Science and Technology, Department of Biotechnology, Ministry of Science and Technology, India, Science and Engineering Research Board