Cytos MPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics
Year: 2026
Type: preprint
Abstract: Cytos Multimodal Pooled Screening (Cytos MPS) measures morphology, protein abundance and localization, CRISPR guide identity, and native 3′ transcriptome in the same fixed cells. Following IL-1β stimulation, cells with higher nuclear NF-κB show lower phospho-p38/HSP27 and higher NFKBIA/DUSP1 in the same cell, a measurement neither imaging nor transcriptomics alone provides. TGFBR2 and CTNNB1 perturbations produce distinct multimodal phenotypes. Guide representation and perturbation transcriptional responses agree with an orthogonal single-cell transcriptomic platform.
Institution: Elements Biosciences (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7214181899