Histological analysis of gingival tissue alteration induced by bisoprolol injection in rats
Year: 2026
Type: article
Abstract: The gingiva, similar to various other tissues, possesses beta-adrenergic receptors, particularly the β2-adrenergic receptor. Gingiva and periodontal ligament fibroblasts express the β2-adrenergic receptor. This in vivo study aimed to investigate for the first time the impact of beta-adrenergic receptor blockers bisoprolol administration on the gingival tissue structure utilizing rat model. Material and methods. A total of 20 Wistar male rats, ages 3 months, were randomly assigned to the control (n=10) and bisoprolol (n=10) groups. A daily subcutaneous injection of 5 mg/kg of bisoprolol was given for two weeks. Upon the end of the study, all of the animals were euthanized, and a full-thickness band of gingival tissue was taken from the palatal aspect of the rats’ molar teeth and routinely prepared for histological and histomorphometric examination. Histomorphometric measurements per region of interest were documented for the following histological variables: Epithelium thickness (from the outer surface to the basement membrane) at, lamina propria thickness, submucosa thickness, and number of blood vessels. Results. Normal distribution of the input data was verified using the Shapiro–Wilk test. Bisoprolol group’s analysis revealed higher values for all variables, yet none achieved a statistical significance for the epithelial thickness (p=0.278), lamina propria thickness (p=0.051), submucosa thickness (p=0.264), and the count of blood vessels (p=0.175). Conclusions: Within the limitations of the current study, the results suggest that the administration of bisoprolol 5mg/kg subcutaneously did not impact on the histological architecture of gingival tissues in rats.
Source: Clinical Dentistry (Russia)
Authors: N.A. Razouki, B.F. Alanbari, H.A. Mahdi
Language: Russian
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7214293375