Reverse Phase High Performance Liquid Chromatography Method Development and Validation for Estimation of Bisoprolol and Hydrochlorothiazide Hcl in Pure and Pharmaceutical Dosage Form
Year: 2026
Type: article
Abstract: A simple, precise, accurate, and robust reverse phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous estimation of Bisoprolol and Hydrochlorothiazide HCl in pure form and pharmaceutical dosage forms. Chromatographic separation was achieved on a C18 column using a suitable mobile phase consisting of an optimized mixture of organic solvent and buffer at a controlled flow rate. Detection was carried out using a UV detector at an appropriate wavelength. The developed method showed good linearity over the selected concentration ranges for both drugs, with correlation coefficients close to unity. The method was validated in accordance with International Council for Harmonisation (ICH) guidelines for parameters such as accuracy, precision, specificity, robustness, limit of detection (LOD), and limit of quantification (LOQ). The percentage recovery studies confirmed the accuracy of the method, while precision studies demonstrated reproducibility. The proposed RP-HPLC method was successfully applied for the routine analysis of Bisoprolol and Hydrochlorothiazide HCl in combined pharmaceutical dosage forms and was found to be suitable for quality control analysis.
Source: IJPAR JOURNAL
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W7214389373