Highly sensitive and selective fluorescent bis-thiosemicarbazone chemosensor for dual recognition of Zn2+ and pyrophosphate anions (P₂O₇4−) with bioimaging applications
Year: 2026
Type: article
Abstract: This study reports the synthesis and characterization of a series of three bis-thiosemicarbazone ligands and their zinc dinuclear complexes, with a focus on 4-tert-butyl-2,6-bis(N-4-allylthiosemicarbazonyl)phenol (H₃L3). This ligand exhibits remarkable photophysical properties and a high analytical performance, acting as a "turn-on" fluorescent sensor for Zn2+ with a significant fluorescence intensity increase via the chelation-enhanced fluorescence (CHEF) mechanism. The [Zn2(L3)(OAc)] complex also functions as a "turn-off" sensor for pyrophosphate (PPi) anions through a ligand displacement mechanism, showing a good selectivity over other anions, with a low detection limit of 3.5 × 10-8 M. The probe combines high sensitivity, signal amplification, and selectivity, making a notably effective sensor among those reported. Importantly, H₃L3 demonstrates a good cell-penetrating ability, enabling in vitro imaging of Zn2+ without significant cytotoxicity under the studied conditions. These findings highlight its potential as a highly effective fluorescent probe for trace-level detection of Zn2+ and advanced analytical applications in complex biological and chemical environments.
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,470
OpenAlex ID: W7214538233