Building an ‘epigenetic clock’: utilizing whole genome DNA methylation patterns to predict age in haddock, Melanogrammus aeglefinus
Year: 2026
Type: article
Abstract: Age-based population models are a gold standard approach to estimate stock size and sustainable catch recommendations for effective fisheries management. Traditional ageing by counting annuli on their ear stones (otoliths), is labor-intensive, lethal, and can be inaccurate for long-lived fishes. In this study, we present an accurate, non-lethal epigenetic clock method for predicting age in haddock, Melanogrammus aeglefinus , one of the most valuable commercial fisheries in the north Atlantic Ocean. Pectoral fin tissue ( n = 140 individuals) was collected during Fall 2022 and Spring 2023 bottom trawl surveys in the Georges Bank-Gulf of Maine region, and whole-genome bisulfite sequencing was conducted to measure CpG loci DNA methylation levels. The most age-predictive loci were identified using a three-step modeling approach: a Bayesian generalized linear mixed model with length and sex as random factors followed by elastic net regression models with a 70/30 train-test split. The final model used 109 loci to predict age with a mean absolute error of 0.17 years. Hypo- and hyper-methylated age-predictive loci were found throughout the genome and were enriched in intragenic regions with functions related to development, immunity, transcriptional regulation, and signal transduction. This study provides an accurate, cost-effective, and scalable epigenetic tool for fisheries management to predict the age of haddock.
Source: Scientific Reports
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Life below water
Open Access status: gold
Estimated APC paid: $2,690
OpenAlex ID: W7214626263
FWCI:
Cites:
Cited by:
Related to: