Work

The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions

Year: 2026

Type: article

Abstract: , the causal agent of kauri dieback, has included soil baiting followed by loop-mediated isothermal amplification (LAMP)-based detection. This approved assay was reported to detect 1 fg of DNA using OptiGene Isothermal Master Mix on a BioRanger instrument. We evaluated its analytical robustness using the same master mix on an alternative platform (QuantStudio 3), and separately tested an alternative NEB master mix. Both assays yielded a reliable detection limit of 1,000 fg, with variable detection at 100 fg and none below. Diagnostic laboratories should verify assay performance under their operational conditions prior to implementation.

Source: PubMed Central

DOI: 10.17912/micropub.biology.002163

Authors: Jade TT Palmer, Monica L Gerth

Language: English

Topic: Plant Pathogens and Resistance

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

Funders: Victoria University of Wellington, New Zealand Plant Protection Society

OpenAlex ID: W7214704484

FWCI:

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Related to: 10