Work

High-throughput droplet screening of filamentous fungi on solid substrates

Year: 2026

Type: article

Abstract: , α-amylase and cellulase) using fluorescence-based activity assays. To this end, we further characterized our top hits through scale-up fungal fermentation to evaluate improvements in both enzyme production and total protein secretion. Our work overcomes a key technical barrier in applying droplet microfluidics to filamentous fungi that allows for high-throughput screening and discovery of new fungal strains that produce active enzymes and heterologous proteins relevant to industrial applications.

Source: RSC Chemical Biology

DOI: 10.1039/d6cb00197a

Authors: Chiara Leal Alves, Jeremy Z. Wu, Elham Khanjani, Adiphol Dilokpimol, Zhiyang Deng, Biplu Prajapati, Niloufar Rahbari, Antti P. Aalto, Mari Valkonen, Steve C.C. Shih

Institutions: Concordia University, VTT Technical Research Centre of Finland

Language: English

Topic: Innovative Microfluidic and Catalytic Techniques Innovation

Subfield: Fluid Flow and Transfer Processes

Field: Chemical Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,962

Funders: Canada Foundation for Innovation, Concordia University, Business Finland, Natural Sciences and Engineering Research Council of Canada

Awards: Bio&Circular Finland — Teknologian tutkimuskeskus VTT Oy (9392/31/2021), G590637829, Microfluidics for synthetic biology

OpenAlex ID: W7214934097

FWCI:

Cites: 55

Cited by:

Related to: 10